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amersham ecl plus reagents  (Cytiva Europe)


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    Structured Review

    Cytiva Europe amersham ecl plus reagents
    Amersham Ecl Plus Reagents, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 15425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+plus+western+blotting+detection+reagents/Amersham+ECL+Western+blot+detection+reagent/pm42813785-279-12-16
    Average 96 stars, based on 15425 article reviews
    amersham ecl plus reagents - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Multi-cohort analysis and experimental study on the function of squalene epoxidase as a ferroptosis inhibitor in HCC
    Article Snippet: .. Finally, protein bands were visualized using ECL Plus Western blotting Detection Reagents (GE Healthcare) and imaged with a chemiluminescence imaging system. .. Band intensities were quantified using ImageJ software.

    Article Title: Phosphate limitation triggers Fe³⁺-dependent polymyxin resistance in Enterobacteriaceae
    Article Snippet: The membrane was blocked with 5% non-fat milk in TBST buffer and was incubated subsequently with monoclonal ANTI-FLAG ® M2 antibody (Sigma) and goat anti-mouse IgG (H+L)-HRP conjugate (Bio-rad). .. To detect FLAG-tagged protein bands, the membrane was submerged in the ECL Plus Western Blotting Detection Reagents (GE Healthcare) for 2 min and then subjected to the imaging system (UVITEC Cambridge) to record signals. ..

    Article Title: Abnormal behaviors and glial responses in an animal model of tau pathology
    Article Snippet: The membrane was incubated with blocking buffer (EveryBlot Blocking Buffer, Cat. #12,010,020; Bio-Rad) for 30 min and then incubated with mouse anti‐Tau monoclonal Antibody (Tau5) (#AHB, diluted 1:500; Invitrogen), rabbit anti–phospho-Tau (Ser404) (#20,194, diluted 1:1000; Cell Signaling Technology, Danvers, MA, USA), rabbit anti–phospho-Tau (Ser214) (#77,348, diluted 1:1000; Cell Signaling), rabbit anti–PSD95(2507S, diluted 1:1000; Cell Signaling), and rabbit anti–synapsin (AB1543P, diluted 1:1000; Millipore) at 4 °C overnight. .. After incubation with a horseradish peroxidase–conjugated anti‐rabbit antibody and a horseradish peroxidase–conjugated anti‐mouse antibody for 2 h, the immune complex was detected using ECL Plus western blotting detection reagents (GE Healthcare, Chalfont St. Giles, Buckinghamshire, UK). ..

    Article Title: Microarray analysis of gene expression in lung tissues of indium-exposed rats: possible roles of S100 proteins in lung diseases.
    Article Snippet: The membrane was incubated with rabbit polyclonal anti-LCN2 antibody (ab63292, 1:1,000, Abcam), rabbit polyclonal anti-S100A9 antibody (ab75478, 1:500, Abcam) or mouse monoclonal anti-β-actin antibody (sc-47778, 1:2,000, an internal standard, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 60 min, and then with horseradish peroxidase-conjugated donkey anti-rabbit IgG or goat anti-mouse IgG antibody (1:10,000 each, Santa Cruz Biotechnology) for 30 min. .. The membrane was treated with ECL plus Western blotting detection reagents (GE Healthcare, Backinghamshire, UK) and analyzed with a LAS-4000 mini biomolecular imager (Fujifilm, Tokyo, Japan). .. We performed quantitative image analysis by measuring the band intensity with an ImageJ software and normalized with β-actin.

    Article Title: Phosphate limitation triggers Fe³⁺-dependent polymyxin resistance in Enterobacteriaceae
    Article Snippet: .. Chemiluminescence of protein bands was detected by ECL Plus Western Blotting Detection Reagents (GE Healthcare, USA), and imaged using a X-ray film development by Alliance Q9 (UVITEC, United Kingdom). ..

    Article Title: Succinate supplementation alleviates liver cancer by inhibiting the FN1/SQLE axis-mediated cholesterol biosynthesis.
    Article Snippet: .. Finally, the membranes containing the interested proteins were visualized using ECL Plus Western blotting Detection Reagents (GE Healthcare). ..

    Article Title: Role of 11β-hydroxysteroid dehydrogenase type 1 inhibition in the antiobesity effect of J2H-1702 on adipocytes and a high-fat diet-induced NASH model.
    Article Snippet: Obesity due to excessive body fat accumulation remains a global problem.. Patients with obesity have high cortisol levels, and its dysregulation is caused by increased 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) levels.. The effects and mechanism of J2H-1702, an 11β-HSD1 inhibitor, on nonalcoholic steatohepatitis (NASH) were explored.

    Article Title: Cell-based assay to detect small molecules restoring levels of let-7 miRNAs
    Article Snippet: Detection was carried out by appropriate horseradish-peroxidase-coupled secondary antibodies [anti-mouse IgG (Thermofisher, 1:5000), anti-rabbit IgG (Santa Cruz Biotechnology, 1:1000). .. ECL plus Western blotting detection reagents (GE Healthcare) were used for visualization according to the manufacturers’ protocol. ..

    Imaging:

    Article Title: Multi-cohort analysis and experimental study on the function of squalene epoxidase as a ferroptosis inhibitor in HCC
    Article Snippet: .. Finally, protein bands were visualized using ECL Plus Western blotting Detection Reagents (GE Healthcare) and imaged with a chemiluminescence imaging system. .. Band intensities were quantified using ImageJ software.

    Article Title: Phosphate limitation triggers Fe³⁺-dependent polymyxin resistance in Enterobacteriaceae
    Article Snippet: The membrane was blocked with 5% non-fat milk in TBST buffer and was incubated subsequently with monoclonal ANTI-FLAG ® M2 antibody (Sigma) and goat anti-mouse IgG (H+L)-HRP conjugate (Bio-rad). .. To detect FLAG-tagged protein bands, the membrane was submerged in the ECL Plus Western Blotting Detection Reagents (GE Healthcare) for 2 min and then subjected to the imaging system (UVITEC Cambridge) to record signals. ..

    Membrane:

    Article Title: Phosphate limitation triggers Fe³⁺-dependent polymyxin resistance in Enterobacteriaceae
    Article Snippet: The membrane was blocked with 5% non-fat milk in TBST buffer and was incubated subsequently with monoclonal ANTI-FLAG ® M2 antibody (Sigma) and goat anti-mouse IgG (H+L)-HRP conjugate (Bio-rad). .. To detect FLAG-tagged protein bands, the membrane was submerged in the ECL Plus Western Blotting Detection Reagents (GE Healthcare) for 2 min and then subjected to the imaging system (UVITEC Cambridge) to record signals. ..

    Article Title: Microarray analysis of gene expression in lung tissues of indium-exposed rats: possible roles of S100 proteins in lung diseases.
    Article Snippet: The membrane was incubated with rabbit polyclonal anti-LCN2 antibody (ab63292, 1:1,000, Abcam), rabbit polyclonal anti-S100A9 antibody (ab75478, 1:500, Abcam) or mouse monoclonal anti-β-actin antibody (sc-47778, 1:2,000, an internal standard, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 60 min, and then with horseradish peroxidase-conjugated donkey anti-rabbit IgG or goat anti-mouse IgG antibody (1:10,000 each, Santa Cruz Biotechnology) for 30 min. .. The membrane was treated with ECL plus Western blotting detection reagents (GE Healthcare, Backinghamshire, UK) and analyzed with a LAS-4000 mini biomolecular imager (Fujifilm, Tokyo, Japan). .. We performed quantitative image analysis by measuring the band intensity with an ImageJ software and normalized with β-actin.

    Incubation:

    Article Title: Abnormal behaviors and glial responses in an animal model of tau pathology
    Article Snippet: The membrane was incubated with blocking buffer (EveryBlot Blocking Buffer, Cat. #12,010,020; Bio-Rad) for 30 min and then incubated with mouse anti‐Tau monoclonal Antibody (Tau5) (#AHB, diluted 1:500; Invitrogen), rabbit anti–phospho-Tau (Ser404) (#20,194, diluted 1:1000; Cell Signaling Technology, Danvers, MA, USA), rabbit anti–phospho-Tau (Ser214) (#77,348, diluted 1:1000; Cell Signaling), rabbit anti–PSD95(2507S, diluted 1:1000; Cell Signaling), and rabbit anti–synapsin (AB1543P, diluted 1:1000; Millipore) at 4 °C overnight. .. After incubation with a horseradish peroxidase–conjugated anti‐rabbit antibody and a horseradish peroxidase–conjugated anti‐mouse antibody for 2 h, the immune complex was detected using ECL Plus western blotting detection reagents (GE Healthcare, Chalfont St. Giles, Buckinghamshire, UK). ..

    Article Title: Role of 11β-hydroxysteroid dehydrogenase type 1 inhibition in the antiobesity effect of J2H-1702 on adipocytes and a high-fat diet-induced NASH model.
    Article Snippet: Obesity due to excessive body fat accumulation remains a global problem.. Patients with obesity have high cortisol levels, and its dysregulation is caused by increased 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) levels.. The effects and mechanism of J2H-1702, an 11β-HSD1 inhibitor, on nonalcoholic steatohepatitis (NASH) were explored.



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